Adequate levels and an appropriate balance of the steroid hormones (estrogens, progesterone, testosterone, DHEA and cortisol) are necessary for maintaining optimal health and well being in both females and males. This family of steroid hormones supports a wide range of essential physiological functions. Variation in these hormones plays a large role in the changes seen in life cycle events such as aging. There has been interest in supporting such hormone activity by dietary changes, herb use, and medications. Quantitative measurement of steroid hormones in saliva allows a non-invasive way to establish a baseline hormone level and monitor the impact of intervention.